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KMID : 0894520090130020105
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Development & Reproduction
2009 Volume.13 No. 2 p.105 ~ p.114
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Effect of Ethane 1,2-Dimethane Sulfonate (EDS) on the Accessory Sex Organs in Adult Rats : A Histological Study
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Lee Won-Yong
Lee Sung-Ho
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Abstract
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Ethane 1,2-dimethane sulfonate (EDS) is a well-known alkylating agent used as selective Leydig cell (LC) toxicant to create a testicular dysfunction model. Previous studies including our own clearly demonstrated the dramatic weight loss of the androgen dependent accessory sex organs such as epididymis, seminal vesicle and prostate gland in this ’LC knock-out’ rats. The present study was performed to evaluate the effect of EDS administration on histological changes of the epididymis, seminal vesicle and prostate in adult rats. Adult male Sprague-Dawley rats (350～400 g B.W.) were injected with a single dose of EDS (75 ㎎/㎏, i.p.) and sacrificed on weeks 0, 1, 2, 3, 4, 5, 6 and 7. Tissue weights (testis, epididymis, seminal vesicle and prostate gland) were measured. The histological changes of tissue were observed by a light microscopy using hematoxylin & eosin staining. Weights of the reproductive and accessory organs progressively declined after the EDS treatments (weeks 1, 2 and 3). After this, the decrease was stopped, then gradually returned to the normal levels. There was a partial (about 60%) recovery of the epididymis weight during weeks 6～7. The cross section of epididymis revealed an increase in thickness of the epithelium during weeks 1～3. In contrast, considerable reduction of epithelial thickness in seminal vesicle was observed during same period. Similarly, a reduction in thickness of prostate epithelial layer was found during weeks 1～3, then it was back to normal thickness after week 4. Taken together, the present study demonstrated that the temporally induced androgen-deficiency by EDS treatment could result the prominent alterations in histology of the accessory sex organs. Further studies on the physiological and molecular regulation of these androgensensitive organs using EDS model will be helpful to understand the normal and pathological development and differentiation mechanism of these organs.
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KEYWORD
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Ethane 1, 2-dimethane sulfonate (EDS), Epididymis, Seminal vesicle, Prostate gland, Histological study
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